Amazing Biotech has innovatively developed a single tube multiplex PCR detection technology and obtained a core technology invention patent. This technology has the following advantages:

Patent 1：Multiple probe melt curve method (MMCM)

Multiple probe melt curve method (MMCM) uses double-labeled oligonucleotide probes to analyze themelt-curve of multiple PCR amplification products. ln a single fluorescence detection channel, the melt curve of two or more double-labeled oligonucleotide probes with the same fluorophore and matched single-stranded nucleic acid products are detected. Enables multiple detection in one fluorescent channel. Using the multi-color fluorescence channel of real-time fluorescent PCR instrument, the number of detection targets is greatly increased.

Patent 2：Amplifying and melting double signal

Based on the principle of Taq enzyme hydrolysis of fluorescent probes to generate fluorescence amplification signals and the principle of melting curve, in the monochrome fluorescence channel, the amplification method is used to detect the detection of one target target while the melting curve is used to detect the other target, thus realizing the simultaneous detection and analysis of two target targets in the monochrome fluorescence channel.

Respiratory Pathogens Gene Kit

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